p nf κb inhibitor α  (Santa Cruz Biotechnology)

Journal: Frontiers in Pharmacology

Article Title: A supercritical oil extract of Schisandra chinensis seeds ameliorates Huntington’s disease-like symptoms and neuropathology: the potential role of anti-oxidant and anti-inflammatory effects

doi: 10.3389/fphar.2024.1471024

Figure Lengend Snippet: SOSC inhibits inflammatory signaling pathways (MAPKs and NF-κB) in the striatum after 3-NPA intoxication. (A–F) Twelve hours after the final (4 th ) 3-NPA intoxication, striatal lysates (n = 5 per group) from Sham, 3-NPA, 3-NPA + SOSC (300 mg/kg/day), and SOSC (300 mg/kg/day) groups were subjected to Western blot assay (A) and quantified (B–F) . SOSC decreased protein expression levels of p-ERK (A, B) , p-JNK (A, C) , p-p38 (A, D) , p-IκB (A and E), and p-NF-κB (A, F) . Data are expressed as mean ± SEM (one-way ANOVA with post hoc test; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. Sham group; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. 3-NPA group). 3-NPA, 3-nitropropionic acid; ERK, extracellular signal-regulated kinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IκB, inhibitor of NF-κB; JNK, Jun amino-terminal kinases; NF-κB, nuclear factor kappa B; SOSC, seed oil of Schisandra chinensis.

Article Snippet: For normalization of antibody signals, membranes were stripped and reprobed with antibodies against glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:5,000; Cell Signaling Technology), beta-Actin (β-Actin; 1:5,000; Santa Cruz Biotechnology) and total IκB/NF-κB p65/JNK/ERK/p38 (1:1,000; Cell Signaling Technology).

Techniques: Protein-Protein interactions, Western Blot, Expressing

Journal: Antioxidants

Article Title: A Combined Extract from Dioscorea bulbifera and Zingiber officinale Mitigates PM 2.5 -Induced Respiratory Damage by NF-κB/TGF-β1 Pathway

doi: 10.3390/antiox13121572

Figure Lengend Snippet: Details of primary and secondary antibodies utilized in this study.

Article Snippet: p-NF-κB inhibitor α (p-IκB-α) , sc-8404 , Santa Cruz Biotech (Dallas).

Techniques:

Journal: Antioxidants

Article Title: A Combined Extract from Dioscorea bulbifera and Zingiber officinale Mitigates PM 2.5 -Induced Respiratory Damage by NF-κB/TGF-β1 Pathway

doi: 10.3390/antiox13121572

Figure Lengend Snippet: Effects of DBZO on inflammation-related protein expression levels in lung tissues of PM 2.5 -exposed BALB/c mice. ( a ) Western blot images, protein expression levels of ( b ) IL-33, ( c ) MyD88, ( d ) p-IκB-α, ( e ) p-NF-κB, ( f ) IL-1β, and ( g ) TNF-α. The results are presented as mean ± SD ( n = 3). Data were statistically considered at p < 0.05, and different small letters represent the statistical differences.

Article Snippet: p-NF-κB inhibitor α (p-IκB-α) , sc-8404 , Santa Cruz Biotech (Dallas).

Techniques: Expressing, Western Blot

Journal: Antioxidants

Article Title: Ecklonia cava Ameliorates Cognitive Impairment on Amyloid β-Induced Neurotoxicity by Modulating Oxidative Stress and Synaptic Function in Institute of Cancer Research (ICR) Mice

doi: 10.3390/antiox13080951

Figure Lengend Snippet: List of primary and secondary antibodies information used in this study.

Article Snippet: p-NF-κB inhibitor alpha (p-IκB-α) , sc-8404 , Santa Cruz Biotech.

Techniques: Binding Assay

Journal: Antioxidants

Article Title: Ecklonia cava Ameliorates Cognitive Impairment on Amyloid β-Induced Neurotoxicity by Modulating Oxidative Stress and Synaptic Function in Institute of Cancer Research (ICR) Mice

doi: 10.3390/antiox13080951

Figure Lengend Snippet: Effect of 70% ethanol extract of Ecklonia cava (EE) on neuroinflammation in Aβ-induced mice. Western blot band images ( a ). Relative expressions of TLR-4 ( b ), MyD88 ( c ), p-IκB-α ( d ), p-NF-κB ( e ), IL-1β ( f ), and TNF-α ( g ) on the corresponding quantitation to β-actin. Data are represented as mean ± SD ( n = 3). Different lowercase letters in the bar graph indicate significant differences between groups ( p < 0.05).

Article Snippet: p-NF-κB inhibitor alpha (p-IκB-α) , sc-8404 , Santa Cruz Biotech.

Techniques: Western Blot, Quantitation Assay